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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC7A11/xCT Recombinant antibody

SLC7A11/xCT Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230227C5

Cat no : 82115-2-RR

Synonyms

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SLC7A11/xCT Recombinant antibody (82115-2-RR)

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SLC7A11/xCT Recombinant antibody (82115-2-RR)

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using SLC7A11/xCT Recombinant antibody (82115-2-RR)

    FC experiment of HeLa using 82115-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using SLC7A11/xCT Recombinant antibody (82115-2-RR)

    FC experiment of A549 using 82115-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

82115-2-RR targets SLC7A11/xCT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC7A11/xCT fusion protein Ag25431 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
Calculated molecular weight 55 kDa
GenBank accession numberBC012087
Gene symbol SLC7A11
Gene ID (NCBI) 23657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

Protocols

Product Specific Protocols
IF protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
FC protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Assist Reprod Genet

PDLIM3 knockdown promotes ferroptosis in endometriosis progression via inducing Gli1 degradation and blocking Hedgehog signaling pathway

Authors - Mingwei Liu
View Article