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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SLC7A11/xCT Recombinant antibody

SLC7A11/xCT Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230227C5

Cat no : CL488-82115-2

Synonyms

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SLC7A11/xCT Recombin (CL488-82115-2)

    IF Staining of HeLa using CL488-82115-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SLC7A11/xCT antibody (CL488-82115-2, Clone: 230227C5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SLC7A11/xCT Recombin (CL488-82115-2)

    FC experiment of HeLa using CL488-82115-2

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated SLC7A11/xCT Recombin (CL488-82115-2)

    FC experiment of A549 using CL488-82115-2

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82115-2 targets SLC7A11/xCT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC7A11/xCT fusion protein Ag25431 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
Calculated molecular weight 55 kDa
GenBank accession numberBC012087
Gene symbol SLC7A11
Gene ID (NCBI) 23657
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SLC7A11/xCT antibody CL488-82115-2Download protocol
Standard Protocols
Click here to view our Standard Protocols