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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC9A9 Monoclonal antibody

SLC9A9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8D9

Cat no : 66577-1-Ig

Synonyms

Na(+)/H(+) exchanger 9, Nbla00118, NHE 9, NHE9, SLC9A9, Sodium/hydrogen exchanger 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC9A9 Monoclonal antibody (66577-1-Ig)

    WB analysis using 66577-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66577-1-Ig (SLC9A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human kidney using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human kidney using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human liver cancer using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human liver cancer using 66577-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IF Staining of HepG2 using 66577-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC9A9 antibody (66577-1-Ig, Clone: 1B8D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66577-1-Ig targets SLC9A9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SLC9A9 fusion protein Ag4660 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 9
Calculated molecular weight 645 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC035779
Gene symbol SLC9A9
Gene ID (NCBI) 285195
RRIDAB_2881937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC9A9 antibody 66577-1-IgDownload protocol
IHC protocol for SLC9A9 antibody 66577-1-IgDownload protocol
IF protocol for SLC9A9 antibody 66577-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols