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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC9A9 Monoclonal antibody, PBS Only

SLC9A9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8D9

Cat no : 66577-1-PBS

Synonyms

Na(+)/H(+) exchanger 9, Nbla00118, NHE 9, NHE9, SLC9A9, Sodium/hydrogen exchanger 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC9A9 Monoclonal antibody (66577-1-Ig)

    WB analysis using 66577-1-Ig (same clone as 66577-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66577-1-Ig (SLC9A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human kidney using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human kidney using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human tonsillitis using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human liver cancer using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IHC staining of human liver cancer using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC9A9 Monoclonal antibody (66577-1-Ig)

    IF Staining of HepG2 using 66577-1-Ig (same clone as 66577-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC9A9 antibody (66577-1-Ig, Clone: 1B8D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66577-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66577-1-PBS targets SLC9A9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SLC9A9 fusion protein Ag4660 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 9
Calculated molecular weight 645 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC035779
Gene symbol SLC9A9
Gene ID (NCBI) 285195
RRIDAB_2881937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.