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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SLK Polyclonal antib (CL488-19743)

    IF Staining of HepG2 using CL488-19743

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SLK antibody (CL488-19743) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated SLK Polyclonal antibody

SLK Polyclonal Antibody for IF/ICC
Cat No. CL488-19743

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

bA16H23.1, CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19743 targets SLK in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name STE20-like kinase (yeast)
Calculated molecular weight 143 kDa
Observed molecular weight 143-160 kDa, 210-220 kDa
GenBank accession numberNM_014720
Gene Symbol SLK
Gene ID (NCBI) 9748
RRIDAB_3672708
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SLK antibody CL488-19743Download protocol
Standard Protocols
Click here to view our Standard Protocols