CoraLite® Plus 488-conjugated SLP76 Monoclonal antibody

SLP76 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H10B1

Cat no : CL488-66465

Synonyms

LCP2, Lymphocyte cytosolic protein 2, SLP 76, SLP 76 tyrosine phosphoprotein, SLP76


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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66465 targets SLP76 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLP76 fusion protein Ag3432 相同性解析による交差性が予測される生物種
Full Name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Calculated molecular weight 533 aa, 60 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC016618
Gene symbol SLP76
Gene ID (NCBI) 3937
RRIDAB_2883339
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SLP76 antibody CL488-66465Download protocol
Standard Protocols
Click here to view our Standard Protocols