CoraLite® Plus 750-conjugated SMAD1 Monoclonal antibody
SMAD1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2D10B8
Cat no : CL750-66559
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-66559 targets SMAD1 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SMAD1 fusion protein Ag0701 相同性解析による交差性が予測される生物種 |
| Full Name | SMAD family member 1 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC001878 |
| Gene symbol | SMAD1 |
| Gene ID (NCBI) | 4086 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 SMAD1 antibody CL750-66559 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
