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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using CoraLite® Plus 750-conjugated SMAD1 Monoclonal ant (CL750-66559)

    WB analysis of HSC-T6 using CL750-66559

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with CL750-66559 (SMAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 750-conjugated SMAD1 Monoclonal antibody

SMAD1 Monoclonal Antibody for WB
Cat No. CL750-66559
Clone No.2D10B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66559 targets SMAD1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMAD1 fusion protein Ag0701 相同性解析による交差性が予測される生物種
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15797
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 SMAD1 antibody CL750-66559Download protocol
Standard Protocols
Click here to view our Standard Protocols