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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD4 Recombinant antibody, PBS Only

SMAD4 Recombinant Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240026G9

Cat no : 83169-5-PBS

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD4 Recombinant antibody (83169-5-RR)

    WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAD4 Recombinant antibody (83169-5-RR)

    IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using SMAD4 Recombinant antibody (83169-5-RR)

    FC experiment of K562 using 83169-5-RR (same clone as 83169-5-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83169-5-PBS is SMAD4 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMAD4 fusion protein Ag0299 相同性解析による交差性が予測される生物種
Full Name SMAD family member 4
Calculated molecular weight 60 kDa
GenBank accession numberBC002379
Gene symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.