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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD4 Recombinant antibody

SMAD4 Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

240026G9

Cat no : 83169-5-RR

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD4 Recombinant antibody (83169-5-RR)

    WB analysis using 83169-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAD4 Recombinant antibody (83169-5-RR)

    IF Staining of HepG2 using 83169-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K562 using SMAD4 Recombinant antibody (83169-5-RR)

    FC experiment of K562 using 83169-5-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, A549 cells, PC-3 cells, MCF-7 cells
Positive IF detected inHepG2 cells
Positive FC detected inK562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83169-5-RR is SMAD4 Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMAD4 fusion protein Ag0299 相同性解析による交差性が予測される生物種
Full Name SMAD family member 4
Calculated molecular weight 60 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC002379
Gene symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.

Protocols

Product Specific Protocols
WB protocol for SMAD4 antibody 83169-5-RRDownload protocol
IF protocol for SMAD4 antibody 83169-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols