Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83169-5 targets SMAD4 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SMAD4 fusion protein Ag0299 相同性解析による交差性が予測される生物種 |
| Full Name | SMAD family member 4 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC002379 |
| Gene Symbol | SMAD4 |
| Gene ID (NCBI) | 4089 |
| RRID | AB_3673212 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13485 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SMAD4 antibody CL488-83169-5 | Download protocol |
| FC protocol for CL Plus 488 SMAD4 antibody CL488-83169-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |