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CoraLite®594-conjugated SMARCA4 Monoclonal antibody
SMARCA4 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2E6B6
Cat no : CL594-66561
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66561 targets SMARCA4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | SMARCA4 fusion protein Ag16256 相同性解析による交差性が予測される生物種 |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
Calculated molecular weight | 1647 aa, 185 kDa |
GenBank accession number | BC150298 |
Gene symbol | SMARCA4 |
Gene ID (NCBI) | 6597 |
RRID | AB_2883603 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 SMARCA4 antibody CL594-66561 | Download protocol |
FC protocol for CL594 SMARCA4 antibody CL594-66561 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |