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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of Jurkat using 66116-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of human brain using 66116-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of HepG2 using 66116-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SMARCD1 Monoclonal antibody

SMARCD1 Monoclonal Antibody for WB, ELISA
Cat No. 66116-1-Ig
Clone No.2F2G6
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66116-1-Ig targets SMARCD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCD1 fusion protein Ag19100 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC009368
Gene Symbol SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2881515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96GM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for SMARCD1 antibody 66116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Eur J Pharmacol

Peptide PDRPS6 attenuates myocardial ischemia injury by improving mitochondrial function

Authors - Mengwen Feng
View Article