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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of Jurkat using 66116-1-Ig (same clone as 66116-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of human brain using 66116-1-Ig (same clone as 66116-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SMARCD1 Monoclonal antibody (66116-1-Ig)

    WB analysis of HepG2 using 66116-1-Ig (same clone as 66116-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

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SMARCD1 Monoclonal antibody, PBS Only

SMARCD1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66116-1-PBS
Clone No.2F2G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66116-1-PBS targets SMARCD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCD1 fusion protein Ag19100 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC009368
Gene Symbol SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2881515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96GM5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.