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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite®594-conjugated SMC1A Monoclonal antibody (CL594-66987)

    IF Staining of human colon cancer using CL594-66987

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 SMC1A antibody (CL594-66987, Clone: 1A5D2 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite®594-conjugated SMC1A Monoclonal antibody

SMC1A Monoclonal Antibody for IF-P
Cat No. CL594-66987
Clone No.1A5D2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF-P

Synonyms

CDLS2, DKFZp686L19178, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66987 targets SMC1A in IF-P applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMC1A fusion protein Ag17257 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 1A
Calculated molecular weight 1233 aa, 143 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC112127
Gene Symbol SMC1A
Gene ID (NCBI) 8243
RRIDAB_2920067
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14683
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Protocols

Product Specific Protocols
IF protocol for CL594 SMC1A antibody CL594-66987Download protocol
Standard Protocols
Click here to view our Standard Protocols