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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMC2 Recombinant antibody, PBS Only

SMC2 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241536E1

Cat no : 84835-2-PBS

Synonyms

SMC protein 2, SMC 2, hCAP E, CAPE, CAP E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC2 Recombinant antibody (84835-2-RR)

    WB analysis using 84835-2-RR (same clone as 84835-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84835-2-RR (SMC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84835-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84835-2-RR

    Affinity and Kinetic Characterization of 84835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84835-2-RR against Human SMC2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84835-2-PBS targets SMC2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMC2 fusion protein Ag33471 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 2
Calculated molecular weight136 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberNM_001042550.1
Gene symbol SMC2
Gene ID (NCBI) 10592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).