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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMC2 Recombinant antibody

SMC2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241536E1

Cat no : 84835-2-RR

Synonyms

SMC protein 2, SMC 2, hCAP E, CAPE, CAP E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC2 Recombinant antibody (84835-2-RR)

    WB analysis using 84835-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84835-2-RR (SMC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84835-2-RR

    Affinity and Kinetic Characterization of 84835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84835-2-RR against Human SMC2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Raji cells, HepG2 cells, A431 cells, HEK-293T cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84835-2-RR targets SMC2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMC2 fusion protein Ag33471 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 2
Calculated molecular weight136 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberNM_001042550.1
Gene symbol SMC2
Gene ID (NCBI) 10592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).

Protocols

Product Specific Protocols
WB protocol for SMC2 antibody 84835-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols