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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC6 Polyclonal antibody (14465-1-AP)

    WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    WB analysis of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SMC6 Polyclonal antibody (14465-1-AP)

    IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:4000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14465-1-AP (SMC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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SMC6 Polyclonal antibody, PBS Only

SMC6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14465-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14465-1-PBS targets SMC6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMC6 fusion protein Ag5812 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 6
Calculated molecular weight 126 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC039828
Gene Symbol SMC6
Gene ID (NCBI) 79677
RRIDAB_2192792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SB8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).