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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMCR7L Monoclonal antibody, PBS Only

SMCR7L Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B3G3

Cat no : 67808-1-PBS

Synonyms

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    WB analysis using 67808-1-Ig (same clone as 67808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IF Staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L/MID51 antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67808-1-PBS targets SMCR7L in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMCR7L fusion protein Ag13775 相同性解析による交差性が予測される生物種
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.