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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMCR7L/MID51 Monoclonal antibody

SMCR7L/MID51 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3B3G3

Cat no : 67808-1-Ig

Synonyms

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    WB analysis using 67808-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IHC staining of mouse testis using 67808-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IHC staining of mouse testis using 67808-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SMCR7L/MID51 Monoclonal antibody (67808-1-Ig)

    IF Staining of mouse testis using 67808-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L/MID51 antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67808-1-Ig targets SMCR7L/MID51 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMCR7L/MID51 fusion protein Ag13775 相同性解析による交差性が予測される生物種
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
RRIDAB_2918571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Protocols

Product Specific Protocols
WB protocol for SMCR7L/MID51 antibody 67808-1-IgDownload protocol
IHC protocol for SMCR7L/MID51 antibody 67808-1-IgDownload protocol
IF protocol for SMCR7L/MID51 antibody 67808-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols