CoraLite® Plus 488-conjugated SMCR7L Monoclonal antibody
SMCR7L Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse , rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B3G3
Cat no : CL488-67808
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67808 targets SMCR7L in IF-P applications and shows reactivity with Human, mouse , rat samples.
| Tested Reactivity | Human, mouse , rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SMCR7L fusion protein Ag13775 相同性解析による交差性が予測される生物種 |
| Full Name | Smith-Magenis syndrome chromosome region, candidate 7-like |
| Calculated molecular weight | 463 aa, 51 kDa |
| Observed molecular weight | 48-51 kDa |
| GenBank accession number | BC002587 |
| Gene symbol | SMCR7L |
| Gene ID (NCBI) | 54471 |
| RRID | AB_2919552 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SMCR7L antibody CL488-67808 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
