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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 488-conjugated SMCR7L Monoclonal an (CL488-67808)

    IF Staining of mouse testis using CL488-67808

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 SMCR7L antibody (CL488-67808, Clone: 3B3G3 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated SMCR7L/MID51 Monoclonal antibody

SMCR7L/MID51 Monoclonal Antibody for IF-P
Cat No. CL488-67808
Clone No.3B3G3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

SMCR7L, Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like, SMCR7-like protein, MIEF1, MID51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67808 targets SMCR7L/MID51 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMCR7L/MID51 fusion protein Ag13775 相同性解析による交差性が予測される生物種
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC002587
Gene Symbol SMCR7L
Gene ID (NCBI) 54471
RRIDAB_2919552
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQG6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SMCR7L/MID51 antibody CL488-67808Download protocol
Standard Protocols
Click here to view our Standard Protocols