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  • Featured Product
  • KD/KO Validated

SMPD1,ASM Polyclonal antibody, PBS Only

SMPD1,ASM Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14609-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMPD1,ASM Polyclonal antibody (14609-1-AP)

    WB analysis using 14609-1-AP (same clone as 14609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14609-1-AP (SMPD1,ASM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SMPD1,ASM Polyclonal antibody (14609-1-AP)

    IHC staining of human brain using 14609-1-AP (same clone as 14609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SMPD1,ASM Polyclonal antibody (14609-1-AP)

    IHC staining of human brain using 14609-1-AP (same clone as 14609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SMPD1,ASM Polyclonal antibody (14609-1-AP)

    IHC staining of human tonsillitis using 14609-1-AP (same clone as 14609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SMPD1,ASM Polyclonal antibody (14609-1-AP)

    IHC staining of human tonsillitis using 14609-1-AP (same clone as 14609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14609-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14609-1-PBS targets SMPD1,ASM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMPD1,ASM fusion protein Ag6180 相同性解析による交差性が予測される生物種
Full Name sphingomyelin phosphodiesterase 1, acid lysosomal
Calculated molecular weight 70 kDa
Observed molecular weight60 kDa, 70-75 kDa
GenBank accession numberBC041164
Gene symbol SMPD1
Gene ID (NCBI) 6609
RRIDAB_2878068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.