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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMYD3 Monoclonal antibody

SMYD3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5C10

Cat no : 66330-1-Ig

Synonyms

SMYD3, ZMYND1, ZNFN3A1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using SMYD3 Monoclonal antibody (66330-1-Ig)

    WB analysis of A431 using 66330-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SMYD3 Monoclonal antibody (66330-1-Ig)

    WB analysis of MCF-7 using 66330-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMYD3 Monoclonal antibody (66330-1-Ig)

    WB analysis of HEK-293 using 66330-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SMYD3 Monoclonal antibody (66330-1-Ig)

    WB analysis of COLO 320 using 66330-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SMYD3 Monoclonal antibody (66330-1-Ig)

    IHC staining of human pancreas cancer using 66330-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMYD3 Monoclonal antibody (66330-1-Ig)

    IHC staining of human liver cancer using 66330-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMYD3 Monoclonal antibody (66330-1-Ig)

    IHC staining of human liver cancer using 66330-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SMYD3 Monoclonal antibody (66330-1-Ig)

    IHC staining of human pancreas cancer using 66330-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SMYD3 Monoclonal antibody (66330-1-Ig)

    IF Staining of MCF-7 using 66330-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SMYD3 antibody (66330-1-Ig, Clone: 1B5C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

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Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66330-1-Ig targets SMYD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMYD3 fusion protein Ag2624 相同性解析による交差性が予測される生物種
Full Name SET and MYND domain containing 3
Calculated molecular weight428aa,49 kDa; 369aa,42 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC031010
Gene symbol SMYD3
Gene ID (NCBI) 64754
RRIDAB_2881711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).

Protocols

Product Specific Protocols
WB protocol for SMYD3 antibody 66330-1-IgDownload protocol
IHC protocol for SMYD3 antibody 66330-1-IgDownload protocol
IF protocol for SMYD3 antibody 66330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols