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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated SMYD3 Monoclonal ant (CL488-66330)

    WB analysis using CL488-66330

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66330 (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 488-conjugated SMYD3 Monoclonal antibody

SMYD3 Monoclonal Antibody for WB
Cat No. CL488-66330
Clone No.1B5C10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

bA74P14.1, FLJ21080, SMYD3, ZMYND1, ZNFN3A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66330 targets SMYD3 in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMYD3 fusion protein Ag2624 相同性解析による交差性が予測される生物種
Full Name SET and MYND domain containing 3
Calculated molecular weight428aa,49 kDa; 369aa,42 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC031010
Gene Symbol SMYD3
Gene ID (NCBI) 64754
RRIDAB_3084234
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H7B4
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) .

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 SMYD3 antibody CL488-66330Download protocol
Standard Protocols
Click here to view our Standard Protocols