CoraLite® Plus 488-conjugated SMYD3 Monoclonal antibody
SMYD3 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B5C10
Cat no : CL488-66330
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66330 targets SMYD3 in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SMYD3 fusion protein Ag2624 相同性解析による交差性が予測される生物種 |
| Full Name | SET and MYND domain containing 3 |
| Calculated molecular weight | 428aa,49 kDa; 369aa,42 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC031010 |
| Gene symbol | SMYD3 |
| Gene ID (NCBI) | 64754 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 SMYD3 antibody CL488-66330 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
