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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00343-2

    Sandwich ELISA standard curve of MP00343-2

    Sandwich ELISA standard curve of MP00343-2, Human SNAI1 Recombinant Matched Antibody Pair - PBS only. 81584-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24248. 81584-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-1000 pg/mL.

  • Sample test of MP00343-2

    Sample test of MP00343-2

    The mean SNAI1 concentration was determined to be 3,819.6 pg/mL in HCT116 cell extract based on a 3.0 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNAI1 Recombinant antibody (81584-4-RR)

    IF Staining of HeLa using 81584-4-RR (same clone as 81584-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNAI1 antibody (81584-4-RR, Clone: 240164C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SNAI1 Recombinant antibody (81584-4-RR)

    FC experiment of A549 using 81584-4-RR (same clone as 81584-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-4-RR, Clone:240164C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00343-2

    Cytometric bead array standard curve of MP00343-2

    Cytometric bead array standard curve of MP00343-2, SNAI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81584-4-PBS. Detection antibody: 81584-3-PBS. Standard: Ag24248. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 81584-4-RR

    Affinity and Kinetic Characterization of 81584-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-4-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

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SNAI1 Recombinant antibody, PBS Only (Capture)

SNAI1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 81584-4-PBS
Clone No.240164C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81584-4-PBS targets SNAI1 as part of a matched antibody pair:

MP00343-2: 81584-4-PBS capture and 81584-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
GenBank accession numberBC012910
Gene Symbol SNAI1
Gene ID (NCBI) 6615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95863
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.