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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNAI1 Recombinant antibody

SNAI1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240164C2

Cat no : 81584-4-RR

Synonyms

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNAI1 Recombinant antibody (81584-4-RR)

    IF Staining of HeLa using 81584-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNAI1 antibody (81584-4-RR, Clone: 240164C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using SNAI1 Recombinant antibody (81584-4-RR)

    FC experiment of A549 using 81584-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-4-RR, Clone:240164C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81584-4-RR

    Affinity and Kinetic Characterization of 81584-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-4-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81584-4-RR is SNAI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
GenBank accession numberBC012910
Gene symbol SNAI1
Gene ID (NCBI) 6615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Protocols

Product Specific Protocols
IF protocol for SNAI1 antibody 81584-4-RRDownload protocol
FC protocol for SNAI1 antibody 81584-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols