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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SNAI1 Recombinant antibody

SNAI1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240164C2

Cat no : CL488-81584-4

Synonyms

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SNAI1 Recombinant an (CL488-81584-4)

    IF Staining of HeLa using CL488-81584-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SNAI1 antibody (CL488-81584-4, Clone: 240164C2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SNAI1 Recombinant an (CL488-81584-4)

    FC experiment of HeLa using CL488-81584-4

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SNAI1 Recombinant antibody (CL488-81584-4, Clone:240164C2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-81584-4 is SNAI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
GenBank accession numberBC012910
Gene symbol SNAI1
Gene ID (NCBI) 6615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SNAI1 antibody CL488-81584-4Download protocol
FC protocol for CL Plus 488 SNAI1 antibody CL488-81584-4Download protocol
Standard Protocols
Click here to view our Standard Protocols