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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAI1 Recombinant antibody (81584-5-RR)

    WB analysis using 81584-5-RR (same clone as 81584-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81584-5-RR (SNAI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81584-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SNAI1 Recombinant antibody (81584-5-RR)

    FC experiment of A549 using 81584-5-RR (same clone as 81584-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-5-RR, Clone:240164C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81584-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81584-5-RR

    Affinity and Kinetic Characterization of 81584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-5-RR against Human SNAI1 were performed. The affinity constant is 0.64 nM.

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SNAI1 Recombinant antibody, PBS Only

SNAI1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81584-5-PBS
Clone No.240164C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81584-5-PBS targets SNAI1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
GenBank accession numberBC012910
Gene Symbol SNAI1
Gene ID (NCBI) 6615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95863
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.