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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNAI1 Recombinant antibody

SNAI1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240164C11

Cat no : 81584-5-RR

Synonyms

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAI1 Recombinant antibody (81584-5-RR)

    WB analysis using 81584-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81584-5-RR (SNAI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using SNAI1 Recombinant antibody (81584-5-RR)

    FC experiment of A549 using 81584-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-5-RR, Clone:240164C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 81584-5-RR

    Affinity and Kinetic Characterization of 81584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-5-RR against Human SNAI1 were performed. The affinity constant is 0.64 nM.

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, BxPC-3 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81584-5-RR is SNAI1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012910
Gene symbol SNAI1
Gene ID (NCBI) 6615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Protocols

Product Specific Protocols
WB protocol for SNAI1 antibody 81584-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols