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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAP91 Polyclonal antibody (14093-1-AP)

    WB analysis using 14093-1-AP (same clone as 14093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14093-1-AP (SNAP91 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IF Staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SNAP91 antibody (14093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

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SNAP91 Polyclonal antibody, PBS Only

SNAP91 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 14093-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14093-1-PBS targets SNAP91 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAP91 fusion protein Ag5224 相同性解析による交差性が予測される生物種
Full Name synaptosomal-associated protein, 91kDa homolog (mouse)
Calculated molecular weight92.5 kDa
Observed molecular weight160 kDa
GenBank accession numberBC060818
Gene Symbol SNAP91
Gene ID (NCBI) 9892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60641
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).