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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SND1 Monoclonal anti (CL488-60265)

    IF Staining of HepG2 using CL488-60265

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated SND1 antibody (CL488-60265, Clone: 1A6A4 ) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated SND1 Monoclonal antibody

SND1 Monoclonal Antibody for IF/ICC
Cat No. CL488-60265
Clone No.1A6A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60265 targets SND1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SND1 fusion protein Ag1200 相同性解析による交差性が予測される生物種
Full Name staphylococcal nuclease and tudor domain containing 1
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC017180
Gene Symbol SND1
Gene ID (NCBI) 27044
RRIDAB_2919240
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7KZF4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SND1 antibody CL488-60265Download protocol
Standard Protocols
Click here to view our Standard Protocols