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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNIP1 Recombinant antibody (82962-1-RR)

    WB analysis using 82962-1-RR (same clone as 82962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNIP1 Recombinant antibody (82962-1-RR)

    IF Staining of HeLa using 82962-1-RR (same clone as 82962-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SNIP1 Recombinant antibody (82962-1-RR)

    FC experiment of U2OS using 82962-1-RR (same clone as 82962-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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SNIP1 Recombinant antibody, PBS Only

SNIP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82962-1-PBS
Clone No.230287D11

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82962-1-PBS targets SNIP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNIP1 fusion protein Ag33543 相同性解析による交差性が予測される生物種
Full Name Smad nuclear interacting protein 1
Calculated molecular weight 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC027040
Gene Symbol SNIP1
Gene ID (NCBI) 79753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAD8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.