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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNIP1 Recombinant antibody

SNIP1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230287D11

Cat no : 82962-1-RR

Synonyms

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNIP1 Recombinant antibody (82962-1-RR)

    WB analysis using 82962-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNIP1 Recombinant antibody (82962-1-RR)

    IF Staining of HeLa using 82962-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using SNIP1 Recombinant antibody (82962-1-RR)

    FC experiment of U2OS using 82962-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HT-29 cells, U2OS cells, BxPC-3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82962-1-RR targets SNIP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNIP1 fusion protein Ag33543 相同性解析による交差性が予測される生物種
Full Name Smad nuclear interacting protein 1
Calculated molecular weight 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC027040
Gene symbol SNIP1
Gene ID (NCBI) 79753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

Protocols

Product Specific Protocols
WB protocol for SNIP1 antibody 82962-1-RRDownload protocol
IF protocol for SNIP1 antibody 82962-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols