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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX18 Monoclonal antibody (68305-1-Ig)

    WB analysis using 68305-1-Ig (same clone as 68305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SNX18 Monoclonal antibody (68305-1-Ig)

    WB analysis of NIH/3T3 using 68305-1-Ig (same clone as 68305-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNX18 Monoclonal antibody (68305-1-Ig)

    IF Staining of A431 using 68305-1-Ig (same clone as 68305-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

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SNX18 Monoclonal antibody, PBS Only

SNX18 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68305-1-PBS
Clone No.2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68305-1-PBS targets SNX18 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX18 fusion protein Ag17388 相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96RF0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).