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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX18 Monoclonal antibody (68305-1-Ig)

    WB analysis using 68305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SNX18 Monoclonal antibody (68305-1-Ig)

    WB analysis of NIH/3T3 using 68305-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNX18 Monoclonal antibody (68305-1-Ig)

    IF Staining of A431 using 68305-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68305-1-Ig
Clone No.2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68305-1-Ig targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX18 fusion protein Ag17388 相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
RRIDAB_2935384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96RF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
WB protocol for SNX18 antibody 68305-1-IgDownload protocol
IF protocol for SNX18 antibody 68305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols