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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E7D6

Cat no : CL488-68305

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated SNX18 Monoclonal ant (CL488-68305)

    IF Staining of A431 using CL488-68305

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 SNX18 antibody (CL488-68305, Clone: 2E7D6 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68305 targets SNX18 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX18 fusion protein Ag17388 相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene symbol SNX18
Gene ID (NCBI) 112574
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SNX18 antibody CL488-68305Download protocol
Standard Protocols
Click here to view our Standard Protocols