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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX3 Monoclonal antibody (68292-1-Ig)

    WB analysis using 68292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68292-1-Ig (SNX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNX3 Monoclonal antibody (68292-1-Ig)

    IF Staining of A431 using 68292-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (68292-1-Ig, Clone: 2B5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SNX3 Monoclonal antibody

SNX3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68292-1-Ig
Clone No.2B5B7

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Synonyms

Grd19, MCOPS8, Protein SDP3, SDP3, SNX3, sorting nexin 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68292-1-Ig targets SNX3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX3 fusion protein Ag1160 相同性解析による交差性が予測される生物種
Full Name sorting nexin 3
Calculated molecular weight 19 kDa
Observed molecular weight16-20 kDa
GenBank accession numberBC008444
Gene Symbol SNX3
Gene ID (NCBI) 8724
RRIDAB_2935372
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60493
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Protocols

Product Specific Protocols
WB protocol for SNX3 antibody 68292-1-IgDownload protocol
IF protocol for SNX3 antibody 68292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols