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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated SNX3 Monoclonal anti (CL488-68292)

    IF Staining of A431 using CL488-68292

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 SNX3 antibody (CL488-68292, Clone: 2B5B7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated SNX3 Monoclonal anti (CL488-68292)

    IF Staining of A431 using CL488-68292

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 SNX3 antibody (CL488-68292, Clone: 2B5B7 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated SNX3 Monoclonal antibody

SNX3 Monoclonal Antibody for IF/ICC
Cat No. CL488-68292
Clone No.2B5B7

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

Grd19, MCOPS8, Protein SDP3, SDP3, SNX3, sorting nexin 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68292 targets SNX3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX3 fusion protein Ag1160 相同性解析による交差性が予測される生物種
Full Name sorting nexin 3
Calculated molecular weight 19 kDa
Observed molecular weight16-20 kDa
GenBank accession numberBC008444
Gene Symbol SNX3
Gene ID (NCBI) 8724
RRIDAB_3084467
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60493
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SNX3 antibody CL488-68292Download protocol
Standard Protocols
Click here to view our Standard Protocols