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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated SNX5 Monoclonal antibody

SNX5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F8A4

Cat no : CL594-67665

Synonyms

Sorting nexin-5, 2F8A4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SNX5 Monoclonal antibody (CL594-67665)

    IF Staining of HeLa using CL594-67665

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated SNX5 antibody (CL594-67665, Clone: 2F8A4 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SNX5 Monoclonal antibody (CL594-67665)

    FC experiment of HeLa using CL594-67665

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SNX5 (CL594-67665, Clone:2F8A4) (red), or 0.4 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67665 targets SNX5 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SNX5 fusion protein Ag12330 相同性解析による交差性が予測される生物種
Full Name sorting nexin 5
Calculated molecular weight 404 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC093623
Gene symbol SNX5
Gene ID (NCBI) 27131
RRIDAB_2920168
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

Protocols

Product Specific Protocols
IF protocol for CL594 SNX5 antibody CL594-67665Download protocol
FC protocol for CL594 SNX5 antibody CL594-67665Download protocol
Standard Protocols
Click here to view our Standard Protocols