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CoraLite®594-conjugated SOCS3 Monoclonal antibody
SOCS3 Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G2B12
Cat no : CL594-66797
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse heart tissue |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66797 targets SOCS3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SOCS3 fusion protein Ag5386 相同性解析による交差性が予測される生物種 |
Full Name | suppressor of cytokine signaling 3 |
Calculated molecular weight | 25 kDa |
Observed molecular weight | 25 kDa |
GenBank accession number | BC060858 |
Gene symbol | SOCS3 |
Gene ID (NCBI) | 9021 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 SOCS3 antibody CL594-66797 | Download protocol |
FC protocol for CL594 SOCS3 antibody CL594-66797 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |