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KD/KO Validated

SOD1 Monoclonal antibody

Name: SOD1 Monoclonal antibody
Brand: Proteintech
SKU: 67480-1-Ig

SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

CloneNo.

2F10G1

Review Product

Cat no : 67480-1-Ig

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis of HeLa using 67480-1-Ig
HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 67480-1-Ig incubated at 1:10000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB analysis using 67480-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of rabbit brain using 67480-1-Ig
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67480-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67480-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 67480-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67480-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67480-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 67480-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 67480-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 67480-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293T using 67480-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of NIH/3T3 using 67480-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of HEK-293T using 67480-1-Ig
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human SOD1 (67480-1-Ig, Clone:2F10G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 67480-1-Ig

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, rabbit brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, K-562 cells, HSC-T6 cells
Positive IHC detected in	human liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HEK-293T cells
Positive FC (Intra) detected in	HEK-293T cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 15 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

67480-1-Ig targets SOD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	SOD1 fusion protein Ag28553 相同性解析による交差性が予測される生物種
Full Name	superoxide dismutase 1, soluble
Calculated molecular weight	16 kDa
Observed molecular weight	16-20 kDa
GenBank accession number	BC001034
Gene symbol	SOD1
Gene ID (NCBI)	6647
RRID	AB_2882707
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643). SOD1 can be detected 24, 32, 40 and 48 kDa by ubiquitination (PMID: 16943203).

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 67480-1-Ig	Download protocol
IHC protocol for SOD1 antibody 67480-1-Ig	Download protocol
IF protocol for SOD1 antibody 67480-1-Ig	Download protocol
FC protocol for SOD1 antibody 67480-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
mouse	IF	Oxid Med Cell Longev Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2 Authors - Jie Jin View Article
rat	WB	Oxid Med Cell Longev Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. Authors - Honglin Liu View Article
human	WB	J Agric Food Chem Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition Authors - Ji-Wang Deng View Article
mouse	WB	Diabetes Metab J Pharmacologic Activation of Angiotensin-Converting Enzyme II Alleviates Diabetic Cardiomyopathy in db/db Mice by Reducing Reactive Oxidative Stress Authors - Donghyun Kim View Article
human,mouse	IF	Biochem Pharmacol Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis Authors - Xinrui Meng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of HeLa using 67480-1-Ig

WB analysis using 67480-1-Ig

WB analysis of rabbit brain using 67480-1-Ig

WB analysis of HeLa using 67480-1-Ig

WB analysis of HEK-293 using 67480-1-Ig

WB analysis of HepG2 using 67480-1-Ig

WB analysis of Jurkat using 67480-1-Ig

WB analysis of pig brain using 67480-1-Ig

WB analysis of rat brain using 67480-1-Ig

WB analysis of mouse brain using 67480-1-Ig

IHC staining of human liver cancer using 67480-1-Ig

IHC staining of human liver cancer using 67480-1-Ig

IHC staining of rat brain using 67480-1-Ig

IHC staining of rat brain using 67480-1-Ig

IHC staining of rat cerebellum using 67480-1-Ig

IHC staining of rat cerebellum using 67480-1-Ig

IHC staining of mouse brain using 67480-1-Ig

IHC staining of mouse brain using 67480-1-Ig

IHC staining of mouse cerebellum using 67480-1-Ig

IHC staining of mouse cerebellum using 67480-1-Ig

IF Staining of HEK-293T using 67480-1-Ig

IF Staining of NIH/3T3 using 67480-1-Ig

FC experiment of HEK-293T using 67480-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2

Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.

Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition

Pharmacologic Activation of Angiotensin-Converting Enzyme II Alleviates Diabetic Cardiomyopathy in db/db Mice by Reducing Reactive Oxidative Stress

Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis