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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F10G1

Cat no : CL594-67480

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated SOD1 Monoclonal antibody (CL594-67480)

    FC experiment of HEK-293T using CL594-67480

    1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOD1 (CL594-67480, Clone:2F10G1) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67480 targets SOD1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOD1 fusion protein Ag28553 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 1, soluble
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC001034
Gene symbol SOD1
Gene ID (NCBI) 6647
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
FC protocol for CL594 SOD1 antibody CL594-67480Download protocol
Standard Protocols
Click here to view our Standard Protocols