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CoraLite® Plus 647-conjugated SOD2 Monoclonal antibody

SOD2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A6C2

Cat no : CL647-66474

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B


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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66474 targets SOD2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOD2 fusion protein Ag21388 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 2, mitochondrial
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
RRIDAB_2920285
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 SOD2 antibody CL647-66474Download protocol
Standard Protocols
Click here to view our Standard Protocols