CoraLite® Plus 488-conjugated SORBS2 Recombinant antibody
SORBS2 Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240023A8
Cat no : CL488-83189-3
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-83189-3 is SORBS2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SORBS2 fusion protein Ag20041 相同性解析による交差性が予測される生物種 |
Full Name | sorbin and SH3 domain containing 2 |
Calculated molecular weight | 1100 aa, 124 kDa |
Observed molecular weight | 70-75 kDa |
GenBank accession number | BC011883 |
Gene symbol | SORBS2 |
Gene ID (NCBI) | 8470 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 SORBS2 antibody CL488-83189-3 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |