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  • KD/KO Validated

Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12369-1-AP

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95


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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12369-1-AP targets Sortilin in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sortilin fusion protein Ag2997 相同性解析による交差性が予測される生物種
Full Name sortilin 1
Calculated molecular weight 831 aa, 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC023542
Gene symbol Sortilin
Gene ID (NCBI) 6272
RRIDAB_2302242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Protocols

Product Specific Protocols
WB protocol for Sortilin antibody 12369-1-APDownload protocol
IHC protocol for Sortilin antibody 12369-1-APDownload protocol
IF protocol for Sortilin antibody 12369-1-APDownload protocol
IP protocol for Sortilin antibody 12369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Evidence for sortilin modulating regional accumulation of human tau prions in transgenic mice.

Authors - Noah R Johnson
humanIF,WB

J Cell Biol

CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.

Authors - Stéphane M Camus
mouseWB,IF

PLoS Pathog

Prions amplify through degradation of the VPS10P sorting receptor sortilin.

Authors - Keiji Uchiyama
  • KO Validated
humanWB

EBioMedicine

Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake.

Authors - Yongtao Xiao
  • KD Validated
humanWB

J Proteome Res

Mapping of the N-linked glycoproteome of human spermatozoa.

Authors - Wang Gaigai G
human,mouseWB,IHC,IF,CoIP

FASEB J

Sortilin acts as an endocytic receptor for α-synuclein fibril

Authors - Shun Ishiyama

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Renato (Verified Customer) (02-28-2019)

Produces a simple clear band and the expected molecular weight. Used as diluent 5% BSA.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue