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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-12369

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    FC experiment of Neuro-2a using CL647-12369

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-12369 targets Sortilin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sortilin fusion protein Ag2997 相同性解析による交差性が予測される生物種
Full Name sortilin 1
Calculated molecular weight 831 aa, 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC023542
Gene symbol Sortilin
Gene ID (NCBI) 6272
RRIDAB_2920221
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Sortilin antibody CL647-12369Download protocol
Standard Protocols
Click here to view our Standard Protocols