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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOX10 Monoclonal antibody, PBS Only (Capture)

SOX10 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat no : 66786-1-PBS

Synonyms

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A375 cells using SOX10 Monoclonal antibody (66786-1-Ig)

    WB analysis of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66786-1-Ig (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IHC staining of mouse brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IHC staining of mouse brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IF Staining of mouse brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SOX10 Monoclonal antibody (66786-1-Ig)

    IF Staining of rat brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C6 cells using SOX10 Monoclonal antibody (66786-1-Ig)

    FC experiment of C6 using 66786-1-Ig (same clone as 66786-1-PBS)

    1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (66786-1-Ig, Clone:1D2C8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50275-1

    Cytometric bead array standard curve of MP50275-1

    Cytometric bead array standard curve of MP50275-1, SOX10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66786-1-PBS. Detection antibody: 66786-2-PBS. Standard:Ag23191. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66786-1-PBS targets SOX10 as part of a matched antibody pair:

MP50275-1: 66786-1-PBS capture and 66786-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOX10 fusion protein Ag23191 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 10
Calculated molecular weight 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC002824
Gene symbol SOX10
Gene ID (NCBI) 6663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)