SOX10 Monoclonal antibody
SOX10 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1D2C8
Cat no : 66786-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells |
Positive IHC detected in | human malignant melanoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue, mouse brain tissue |
Positive FC (Intra) detected in | C6 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 3 publications below |
IF | See 5 publications below |
Product Information
66786-1-Ig targets SOX10 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, chicken |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | SOX10 fusion protein Ag23191 相同性解析による交差性が予測される生物種 |
Full Name | SRY (sex determining region Y)-box 10 |
Calculated molecular weight | 50 kDa |
Observed molecular weight | 55-60 kDa |
GenBank accession number | BC002824 |
Gene symbol | SOX10 |
Gene ID (NCBI) | 6663 |
RRID | AB_2882131 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)
Protocols
Product Specific Protocols | |
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WB protocol for SOX10 antibody 66786-1-Ig | Download protocol |
IHC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
IF protocol for SOX10 antibody 66786-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Elife Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve. | ||
Physiol Behav Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria. | ||
J Neurosci Methods Characterization of the GfaABC1D promoter to selectively target astrocytes in the rhesus macaque brain. | ||
Res Sq Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sonia (Verified Customer) (07-24-2019) | Works fine in longitudinal section of mouse sciatic nerve
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FH Crystal (Verified Customer) (02-19-2019) | Works great in chicken embryos! We used 1:500-1:1000 dilution for the primary and made sure to wash it extensively between primary and secondary.
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