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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1D2C8

Cat no : CL555-66786

Synonyms

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C6 cells using CoraLite®555-conjugated SOX10 Monoclonal antibody (CL555-66786)

    FC experiment of C6 using CL555-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOX10 (CL555-66786, Clone:1D2C8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66786 targets SOX10 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOX10 fusion protein Ag23191 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 10
Calculated molecular weight 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC002824
Gene symbol SOX10
Gene ID (NCBI) 6663
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Protocols

Product Specific Protocols
FC protocol for CL555 SOX10 antibody CL555-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols