Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-66786 targets SOX10 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SOX10 fusion protein Ag23191 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 10 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC002824 |
| Gene Symbol | SOX10 |
| Gene ID (NCBI) | 6663 |
| RRID | AB_2934678 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56693 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 SOX10 antibody CL555-66786 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |