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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated SOX2 Monoclonal anti (CL488-66411)

    IF Staining of mouse brain using CL488-66411

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66411 (SOX2 antibody) at dilution of 1:50.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated SOX2 Monoclonal antibody

SOX2 Monoclonal Antibody for IF-P
Cat No. CL488-66411
Clone No.4B9B10

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

IF-P

Synonyms

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66411 targets SOX2 in IF-P applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX2 fusion protein Ag13635 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 2
Calculated molecular weight 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
RRIDAB_2883330
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48431
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development.. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SOX2 antibody CL488-66411Download protocol
Standard Protocols
Click here to view our Standard Protocols