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SOX9 Monoclonal antibody
SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (4)
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1H9A6
Cat no : 67439-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, Caco-2 cells, human placenta tissue, NCCIT cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 45 publications below |
| IHC | See 1 publications below |
| IF | See 9 publications below |
| FC | See 1 publications below |
Product Information
67439-1-Ig targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SOX9 fusion protein Ag28620 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 9 |
| Calculated molecular weight | 509 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC007951 |
| Gene symbol | SOX9 |
| Gene ID (NCBI) | 6662 |
| RRID | AB_2882675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX9 antibody 67439-1-Ig | Download protocol |
| FC protocol for SOX9 antibody 67439-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology | ||
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI | ||
Mater Today Bio Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction | ||
J Nanobiotechnology Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy | ||
Free Radic Biol Med Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes | ||
EBioMedicine Decreased miR-214-3p activates NF-κB pathway and aggravates osteoarthritis progression. |
